Best pharmazie podcasts we could find (Updated December 2018)
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In order to understand in more depth and on a genome wide scale the behavior of transcription factors (TFs), novel quantitative experiments with high-throughput are needed.Recently, HiTS-FLIP (High-Throughput Sequencing-Fluorescent Ligand Interaction Profiling) was invented by the Burge lab at the MIT (Nutiu et al. (2011)). Based on an Illumina ...…
 
In the present study, the structure and mechanism of two assembly chaperones of Rubisco, Raf1 and RbcX, were investigated. The role of Raf1 in Rubisco assembly was elucidated by analyzing cyanobacterial and plant Raf1 with a vast array of biochemical and biophysicaltechniques. Raf1 is a dimeric protein. The subunits have a two-domain structure. ...…
 
Phenylketonuria (PKU) is an autosomal recessive inborn error of metabolism (IEM) caused by mutations in the phenylalanine hydroxylase (PAH) gene. The molecular mechanism underlying deficiency of the PAH protein is, in most of the cases, loss of function due to protein misfolding. PAH mutations induce disturbed oligomerisation, decreased stabili ...…
 
FK506 binding protein 5 (FKBP5) has been linked to stress related diseases and treatment response in depression (Binder et al., 2004). The corresponding protein FKBP51 was first identified as co-chaperone of HSP90 in a complex with steroid hormone receptors, where it diminishes hormone affinity and nuclear translocation efficiency of the recept ...…
 
The tetraspanin peripherin-2 is a glyco-membrane protein exclusively expressed in the outer segments of rod and cone photoreceptors. Mutations in peripherin-2 are associated with retinal disorders characterized by Degeneration of rod or cone cells. Previous unpublished work identified peripherin-2 as a potential novel part of the protein comple ...…
 
Fluorescence microscopy is a widely used technique for imaging of biological structures due to its noninvasiveness although resolution of conventional fluorescence microscopes is limited to about 200-300 nm due to the diffraction limit of light. Super-resolution fluorescence microscopy offers an extension of the original method that allows opti ...…
 
Endothelial cell (EC) migration is an essential process in angiogenesis as ECs sprout from preexisting vessels, following chemotactic gradients. However, most of the data obtained about EC migration has been acquired in artificial two dimensional (2D) cell culture environments. Recent reports showed that migration in fibrillary environments can ...…
 
Eukaryotic genomes are organized inside the cell nucleus in a structured macromolecularDNA-protein polymer named chromatin, formed by single discrete unites called Nucleosomes.The packing of the genetic information into chromatin allows the efficient regulation of several nuclear processes, such as gene expression and transcription, DNA replica ...…
 
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