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Episode 152: Evaluation of FPDM for STA1 Positive (Diastaticus) Yeast in the Brewery
Manage episode 247337123 series 1265506
Saccharomyces cerevisiae var. diastaticus is a beer spoilage yeast, with contamination leading to off-flavors, over-attenuation, and over-carbonation, potentially causing gushing beer, exploding packages, or non-compliance with Alcohol by Volume reporting. Many diastaticus yeast are commercially available, high-attenuating strains, but wild strains have also been isolated in beer. Regardless of the source of contamination, re-fermentation of finished beer by diastaticus is caused by the secretion of a glucoamylase. In finished beer, glucoamylase breaks down unfermented dextrins, creating newly fermentable carbohydrates. Outside of PCR-based genetic screening, there exists no easy method for detection of diastaticus contamination by the brewery quality control lab. Matt Linske joins us live from the 2019 Master Brewers Conference to discuss his evaluation of Farber Pham Diastaticus Medium (FPDM) for the enrichment and detection of diastaticus, and provides tips for effective use of FPDM in the brewery.
Special Guest: Matt Linske.
Sponsored By:
Links:
- 2019 Master Brewers Conference Proceedings — New member benefit!
- Calgary Presentation 41 - Evaluation of FPDM for the detection of diastaticus in the brewery
- EP 068: Diastaticus - Part 1
- EP 069: Diastaticus - Part 2
- Contact your District Officers — (and offer to help get those district presentations uploaded!)
- Suggest a district presentation we should highlight on the show
- Upcoming Events - Master Brewers Calendar
- 2020 World Brewing Congress
336 episodes
Manage episode 247337123 series 1265506
Saccharomyces cerevisiae var. diastaticus is a beer spoilage yeast, with contamination leading to off-flavors, over-attenuation, and over-carbonation, potentially causing gushing beer, exploding packages, or non-compliance with Alcohol by Volume reporting. Many diastaticus yeast are commercially available, high-attenuating strains, but wild strains have also been isolated in beer. Regardless of the source of contamination, re-fermentation of finished beer by diastaticus is caused by the secretion of a glucoamylase. In finished beer, glucoamylase breaks down unfermented dextrins, creating newly fermentable carbohydrates. Outside of PCR-based genetic screening, there exists no easy method for detection of diastaticus contamination by the brewery quality control lab. Matt Linske joins us live from the 2019 Master Brewers Conference to discuss his evaluation of Farber Pham Diastaticus Medium (FPDM) for the enrichment and detection of diastaticus, and provides tips for effective use of FPDM in the brewery.
Special Guest: Matt Linske.
Sponsored By:
Links:
- 2019 Master Brewers Conference Proceedings — New member benefit!
- Calgary Presentation 41 - Evaluation of FPDM for the detection of diastaticus in the brewery
- EP 068: Diastaticus - Part 1
- EP 069: Diastaticus - Part 2
- Contact your District Officers — (and offer to help get those district presentations uploaded!)
- Suggest a district presentation we should highlight on the show
- Upcoming Events - Master Brewers Calendar
- 2020 World Brewing Congress
336 episodes
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