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Micro binfie podcast

Microbial Bioinformatics

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Microbial Bioinformatics is a rapidly changing field marrying computer science and microbiology. Join us as we share some tips and tricks we’ve learnt over the years. If you’re student just getting to grips to the field, or someone who just wants to keep tabs on the latest and greatest - this podcast is for you. The hosts are Dr. Lee Katz from the Centres for Disease Control and Prevention (US), Dr. Nabil-Fareed Alikhan and Dr. Andrew Page both from Quadram Institute Bioscience (UK) and brin ...
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In this episode of the Micro binfie Podcast, host Andrew Page sits down with Tim Dallman at the 10th Bioinformatics Hackathon in Bethesda, Maryland. Tim shares insights from his work at Utrecht University in the Netherlands, where he focuses on genomic surveillance and machine learning models to predict disease risk and severity. They discuss the c…
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Host Andrew Page is joined by Robert Petit from the Wyoming Public Health Laboratory. Robert, a key developer of the Bactopia pipeline, shares insights into how this end-to-end tool is transforming bacterial genomic surveillance. They dive into the origins of Bactopia, its applications in public health, and Robert's experience leading genomic proje…
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Andrew and Lee talk with Christine and Cynney about the Haiti cholera outbreakCynney Walters: https://www.linkedin.com/in/cynney-walters-763111190Walters et al, "Genome sequences from a reemergence of Vibrio cholerae in Haiti, 2022 reveal relatedness to previously circulating strains" https://journals.asm.org/doi/abs/10.1128/jcm.00142-23…
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We go over tree visualizations!* Microreact https://microreact.org/,https://www.phylocanvas.gl/ * grapetree https://github.com/achtman-lab/GrapeTree* Auspice/NextStrain https://nextstrain.org/* Taxonium https://taxonium.org/* Itol: https://itol.embl.de/ * PhyloViz https://online.phyloviz.net/index* Phandango https://jameshadfield.github.io/phandang…
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In this episode of the Micro Binfie Podcast, hosts Dr. Andrew Page and Dr. Lee Katz delve into the fascinating world of hash databases and their application in cgMLST (core genome Multilocus Sequence Typing) for microbial bioinformatics.The discussion begins with the challenges faced by bioinformaticians due to siloed MLST databases across the glob…
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We discuss GAMBIT, software for accurately classifying bacteria and eukaryotes using a targeted k-mer based approach. GAMBIT software: https://github.com/gambit-suite/gambitGAMBIT suite: https://github.com/gambit-suiteGAMBIT (Genomic Approximation Method for Bacterial Identification and Tracking): A methodology to rapidly leverage whole genome sequ…
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In this episode, Andrew Page and Lee Katz continue their conversation with Titus Brown, diving deeper into his work on k-mers, Sourmash, and open source software development:Topics discussed:K-mers for analyzing sequencing data, and how Sourmash builds on MinHashHow Sourmash handles k-mers for metagenomic comparisons vs. MASHThe modhash and bottom …
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In this final episode with Titus Brown, the conversation focuses on his work scaling metagenomic search with Sourmash:An overview of what Sourmash does - sketching and comparing large k-mer datasetsHow the sampling approach enables analyses like containment estimationExciting capabilities of the Branchwater tool for multi-threaded real-time SRA sea…
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In this episode, Andrew Page and Lee Katz continue their chat with Titus Brown, focusing on taxonomy assignment in metagenomics:Topics discussed:Dealing with contamination and low quality genomes in reference databasesSourmash as a versatile search tool, not a curated databaseThe need for high confidence in taxonomic assignment in public healthMost…
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Andrew, Nabil, and Lee react to the bioinformatics and the science overall in the 1993 film Jurassic Park.We looked at these YouTube clips:* https://youtu.be/mDTaykXudVI?si=I5aiUdBGStpIKHVC* https://youtu.be/RLz5Api676Y?si=D6nps33O42Fmk4Ac* https://youtu.be/0Nz8YrCC9X8?si=KNwkeFoS6Bu4LOpv* https://youtu.be/dxIPcbmo1_U?si=TOBw5AONYVCW0JzV* https://y…
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In this episode of the Micro Binfie Podcast, Andrew Page and Lee Katz interview Titus Brown about his journey from studying math and physics as an undergrad to becoming a bioinformatician focusing on metagenomics and software development.Topics discussed:Titus' background in math, physics, digital evolution research, and developmental biologyHis tr…
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The podcast discusses an article co-authored by Andrew Page, examining the use of GPT-4 for research publication. The conversation focuses on the authorship of articles generated by GPT-4 and the implications for academic publishing.Authorship and Ethics:Andrew discusses the question of authorship when AI-generated content is involved in research a…
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We continue our conversation with Wytamma Wirth about write-the and all things AI. It starts with discussing the usage of language models, specifically ChatGPT, in writing boilerplate code, and how it can assist in generating code snippets, unit tests, and even documentation strings. The participants also explore the potential of incorporating it i…
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In this episode, we dive deep into the world of automated code documentation and conversion using ChatGPT through the write-the software developed by Dr Wytamma Wirth from The University of Melbourne. Our guest, an experienced software engineer, takes us on a journey through the challenges and nuances of writing code documentation and the role AI c…
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In this episode there is a comprehensive discussion on the influence of AI, especially GPT-4, in the sphere of microbial bioinformatics. They reflect on a study testing GPT-4's problem-solving capabilities, which raises concerns about its potential impact on employment practices and academic integrity.There's speculation that AI's proficiency in ta…
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In this episode of the Micro Binfie Podcast, titled "AI Unleashed: Navigating the Opportunities and Challenges of AI in Microbial Bioinformatics", Lee, Nabil, and Andrew unpack the implications of generative predictive text AI tools, notably GPT, on microbial bioinformatics. They kick off the conversation by outlining the various applications of AI…
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The MicroBinfie podcast discusses the top programming languages for bioinformatics. Andrew, Lee, and Nabil agree that Python is a great starting point for its consistency and rigor. Its strict syntax is ideal for teaching programming fundamentals that are essential in any language. In contrast, Perl encourages multiple ways of doing the same thing,…
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We are back talking about systematics, and SeqCode; a nomenclatural code for prokaryotes described from sequence data. Marike Palmer is a Postdoctoral researcher in the School of Life Sciences at the University of Nevada Las Vegas and Miguel Rodriguez is an Assistant Professor of Bioinformatics at the University of Innsbruck in the departments of M…
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Today we are talking about systematics, and specifically SeqCode; a nomenclatural code for prokaryotes described from sequence data. Joining us to talk about it are co-authors on the recent publication. Marike Palmer and Miguel Rodriguez. Marike Palmer is a Postdoctoral researcher in the School of Life Sciences at the University of Nevada Las Vegas…
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An honest discussion about the up and downsides of doing a postdoc in front of an audience of first year PhD students. Guests Dr Emma Waters, Dr Heather Felgate and Dr Muhammad Yasir are joined by Dr Andrew Page.It was recorded in front of a live audience of PhD students at the Microbes, Microbiomes and Bioinformatics doctoral training program in t…
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This is a panel discussion on mobile genetic elements, guest chaired by Dr Muhammad Yasir with guests Dr Emma Waters, Dr Heather Felgate and Dr Andrew Page. We cover AMR, Salmonella Typhi and Staphylococci and outbreaks and the role of MGEs.It was recorded in front of a live audience of PhD students at the Microbes, Microbiomes and Bioinformatics d…
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We talk about KRAKEN the taxonomic classification software and the software suite around it and are joined by Jennifer Lu and Natalia Rincon from Johns Hopkins University Center for Computational Biology.Dr. Jennifer Lu and Natalia Rincon from the Kraken software development team were interviewed on the MicroBinfie podcast. They discussed the vario…
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We are talking about KRAKEN - the taxonomic classification software and in the hot seat are Dr Jennifer Lu and Natalia Rincon from Johns Hopkins University Center for Computational Biology.The MicroBinfie podcast welcomed Dr. Jennifer Lu and Natalia Rincon to discuss Kraken, a taxonomic classification software. Developed in 2013-2014, Kraken easily…
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Ed Feil is a professor of bacterial evolution at the University of Bath, and Natacha Couto, a data scientist at the Center of Genomic Pathogen Surveillance at the University of Oxford. We delve into the concept of multi-locus sequence typing (MLST) in bacterial population genetics.They highlight how the MLST method allows for defining strains based…
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The hosts of the MicroBinfie podcast invite Dr Natacha Couto (University of Oxford) and Professor Ed Feil (University of Bath) as special guests to discuss the concept of "One Health". One Health is a comprehensive approach that seeks to manage the problem of antimicrobial resistance (AMR) by addressing the use of antibiotics in healthcare, agricul…
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We celebrate having 100 episodes! We look back at the history of our podcast and then talk about what the future might hold.Then: Lee gets his revenge by having Andrew and Nabil pronounce words local to him.We very briefly mentioned this paper: https://www.nature.com/articles/s41586-022-05543-xNabil was trying to remember this particular site and r…
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At the 8th Microbial Bioinformatics Hackathon in Bath we talked to a live panel with Kristy Horan, Torsten Seemann, Finlay Maguire and Andrew Page about bioinformatics from the frontlines. We apologise for the poor audio quality, it was recorded in a room with 20 people in the background so at points it got a bit loud, however we felt you might enj…
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We interview Frank Ambrosio. He is embarking on a lifestyle of nomadic bioinformatics, living his best life.* https://www.linkedin.com/in/francis-ambrosio/In this episode of the MicroBinfie podcast, Frank Ambrosio, a bioinformatician working for Theiagen as a traveling bioinformatician, joins co-hosts Andrew Nabil and Lee to talk about his journey …
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We discuss recent advancements in genome sequencing technologies, based on what we've been hearing at conferences and within the community.The Microbial Bioinformatics podcast brought together three experts, Andrew, Lee, and Nabil, to discuss the latest advances in sequencing technologies. The team explored the new developments in the market, inclu…
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For the first time ever all 3 MicroBinfies are together in person to record an episode. We are joined by Torsten Seemann for a conversation about how what we do in research can get lost in translation when applied to public health. We discuss what we did with SARS-CoV-2 genomics and somehow end up chatting about geography and language. Hope you enj…
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Over the past few weeks scientists have been swapping Twitter for Mastodon. Our very own Nabil-Fareed Alikhan talks about his experience with setting up and running a Mastodon server called https://mstdn.science which is one of the places where scientists have moved over to. We are joined by Emma Hodcroft to get an independent scientists view on th…
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Often the hard part of bioinformatics isnt the analysis, its getting all of the software you need setup and installed. Come with us on this journey and avoid dependancy hell.In the MicroBinfie podcast, the hosts discuss the struggles of installing, managing, and dealing with dependencies with bioinformatics software. In the past, software installat…
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The MicroBinfie podcast discusses the top programming languages for bioinformatics. Andrew, Lee, and Nabil agree that Python is a great starting point for its consistency and rigor. Its strict syntax is ideal for teaching programming fundamentals that are essential in any language. In contrast, Perl encourages multiple ways of doing the same thing,…
  continue reading
 
Today on the @microbinfie podcast, we talk about WDL with @sevinsky and @DannyJPark. We learn what widdle means to Andrew and his kids. Joel takes a shot at Lyve-SET and you'll never guess what happens next.In the MicroBinfie podcast, we discuss the workflow description language (WDL) commonly used to describe bioinformatics pipelines in a portable…
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This is an extended directors cut of our chat with Dr Henk den Bakker about Sepia. Its a summer holiday bonus.Some URLsGet Sepia here: https://github.com/hcdenbakker/sepiaSome information on the food safety informatics group at UGA: https://www.denglab.site/Rust: https://www.rust-lang.org/Kalamari: https://github.com/lskatz/kalamariCAMI: https://ww…
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We are joined again by Prof Mark Pallen who takes us through his early experiences in high-throughput microbial genomics. Mark was pleased that he persuaded Nick Loman to join him in Birmingham. Mark tells us how they worked with George Weinstock to perform the first genome sequence analyses of Gram-negatives for genomic epidemiology—in this case o…
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Mark Pallen explains how exciting it was to be in microbial bioinformatics around the turn of the millennium, as we gained genomes for the first time from model organisms and fearsome pathogens. He recounts working with his hero David Relman on the genome sequencing of the strange slow-growing organism called Tropheryma whipplei in competition with…
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In this episode we talk to Professor Mark Pallen, who discusses the highlights from his long career as a medical microbiologist turned bioinformatician. His bioinformatics journey began in 1977, the year Fred Sanger invented DNA sequencing-as-we know-it, when Mark was tasked with assembling some amino acid sequences under exam conditions. Mark expl…
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