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Back to Buffer Basics: Everything You Need to Know About Buffers

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Manage episode 410030660 series 3391756
Content provided by Bitesize Bio. All podcast content including episodes, graphics, and podcast descriptions are uploaded and provided directly by Bitesize Bio or their podcast platform partner. If you believe someone is using your copyrighted work without your permission, you can follow the process outlined here https://player.fm/legal.

Buffers are critical to nearly all our experiments. Poorly prepared buffers lead to failed Western blots, poor protein yields, flat binding curves—and wasted time.

Understanding why you need buffers, knowing how to choose the right one, and troubleshooting buffer issues are fundamental skills you can apply to make all your experiments work better—and you only need to learn how to prepare them correctly once.

In this episode, dive deep into the world of chemical buffers. Explore how to select the best buffer for your experiments, share practical tips on preparing buffer solutions, and troubleshoot any hiccups that might come your way. Plus, we'll demystify key concepts like pH and pKa.

Watch the full presentation here: https://events.bitesizebio.com/back-to-buffer-basics-everything/join

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

  continue reading

113 episodes

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iconShare
 
Manage episode 410030660 series 3391756
Content provided by Bitesize Bio. All podcast content including episodes, graphics, and podcast descriptions are uploaded and provided directly by Bitesize Bio or their podcast platform partner. If you believe someone is using your copyrighted work without your permission, you can follow the process outlined here https://player.fm/legal.

Buffers are critical to nearly all our experiments. Poorly prepared buffers lead to failed Western blots, poor protein yields, flat binding curves—and wasted time.

Understanding why you need buffers, knowing how to choose the right one, and troubleshooting buffer issues are fundamental skills you can apply to make all your experiments work better—and you only need to learn how to prepare them correctly once.

In this episode, dive deep into the world of chemical buffers. Explore how to select the best buffer for your experiments, share practical tips on preparing buffer solutions, and troubleshoot any hiccups that might come your way. Plus, we'll demystify key concepts like pH and pKa.

Watch the full presentation here: https://events.bitesizebio.com/back-to-buffer-basics-everything/join

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

  continue reading

113 episodes

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