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Episode 06 - A Pretty Fly DNA Extraction Method

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Content provided by Dr Jonny Coates. All podcast content including episodes, graphics, and podcast descriptions are uploaded and provided directly by Dr Jonny Coates or their podcast platform partner. If you believe someone is using your copyrighted work without your permission, you can follow the process outlined here https://player.fm/legal.

This week we discuss how to non-destructively extract and sequence DNA from historical mosquito and tsetse fly samples from the Natural History Museum’s collection. We discover a new method for doing just that with Dr Petra Korlevic (@petrathepostdoc), a Post-doctoral researcher at the EMBL-EBI (@emblebi)/Sanger Institute (@sangerinstitute), and Dr Erica McAlister (@flygirlNHM), Senior Curator of Diptera at the Natural History Museum (@NHM_London). Erica and Petra explain how this method can be used to open up museum collections to molecular researchers in order to answer questions around subjects such as the rise of insecticide resistance amongst these disease vector insects, and the diet, behaviour and ecology of key pollinator species during the rise of global warming or changes in farming practice. We talk about the benefits of preprinting, and how digitising museum collections and developing molecular methods such as this, is increasing the accessibility of data on the specimens within them to scientists around the world.

Read the full preprint https://www.biorxiv.org/content/10.1101/2021.06.28.450148v1.full

Other links

https://www.nhm.ac.uk/our-science/collections/entomology-collections.html

https://www.nhm.ac.uk/our-science/departments-and-staff/life-sciences/insects.html

https://www.nhm.ac.uk/our-science/our-work/digital-collections/digital-collections-programme.html

https://www.liverpoolmuseums.org.uk/insect-collections

This episode was produced by John D Howard & Emma Wilson and edited by John D Howard.

If you enjoyed this show then hit that subscribe button and leave a review. If you love what we are trying to do then support us on Patreon https://www.patreon.com/preprintsinmotion where tiers start at as little as £1 a month!

For the latest podcast news and updates follow us on Twitter @MotionPod or visit our website; www.preprintsinmotion.com.

Produced by JEmJ Productions (find us on Twitter: Jonny @JACoates, Emma @ELWilson92, John @JohnDHoward8) and generously supported by ASAPbio (https://asapbio.org | @asapbio_).

  continue reading

51 episodes

Artwork
iconShare
 
Manage episode 297967196 series 2942968
Content provided by Dr Jonny Coates. All podcast content including episodes, graphics, and podcast descriptions are uploaded and provided directly by Dr Jonny Coates or their podcast platform partner. If you believe someone is using your copyrighted work without your permission, you can follow the process outlined here https://player.fm/legal.

This week we discuss how to non-destructively extract and sequence DNA from historical mosquito and tsetse fly samples from the Natural History Museum’s collection. We discover a new method for doing just that with Dr Petra Korlevic (@petrathepostdoc), a Post-doctoral researcher at the EMBL-EBI (@emblebi)/Sanger Institute (@sangerinstitute), and Dr Erica McAlister (@flygirlNHM), Senior Curator of Diptera at the Natural History Museum (@NHM_London). Erica and Petra explain how this method can be used to open up museum collections to molecular researchers in order to answer questions around subjects such as the rise of insecticide resistance amongst these disease vector insects, and the diet, behaviour and ecology of key pollinator species during the rise of global warming or changes in farming practice. We talk about the benefits of preprinting, and how digitising museum collections and developing molecular methods such as this, is increasing the accessibility of data on the specimens within them to scientists around the world.

Read the full preprint https://www.biorxiv.org/content/10.1101/2021.06.28.450148v1.full

Other links

https://www.nhm.ac.uk/our-science/collections/entomology-collections.html

https://www.nhm.ac.uk/our-science/departments-and-staff/life-sciences/insects.html

https://www.nhm.ac.uk/our-science/our-work/digital-collections/digital-collections-programme.html

https://www.liverpoolmuseums.org.uk/insect-collections

This episode was produced by John D Howard & Emma Wilson and edited by John D Howard.

If you enjoyed this show then hit that subscribe button and leave a review. If you love what we are trying to do then support us on Patreon https://www.patreon.com/preprintsinmotion where tiers start at as little as £1 a month!

For the latest podcast news and updates follow us on Twitter @MotionPod or visit our website; www.preprintsinmotion.com.

Produced by JEmJ Productions (find us on Twitter: Jonny @JACoates, Emma @ELWilson92, John @JohnDHoward8) and generously supported by ASAPbio (https://asapbio.org | @asapbio_).

  continue reading

51 episodes

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