The Listen In podcast from Bitesize Bio is a compilation of our best webinars to enjoy at your leisure, wherever and whenever. Each episode is an opportunity to gain the valuable insights you need to advance your research. From a crash course in developing fool-proof ELISAs to the latest applications and innovations in CRISPR/Cas9 and microscopy techniques, and much more—you'll hear about challenges encountered and discover practical solutions to achieve the best possible results. Tap into t ...
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Visualizing Protein-Protein Interactions by Non-fitting and Easy FRET-FLIM Approaches
1:10:53
1:10:53
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Understanding molecular interactions in living cells is one of the key elements to deciphering the molecular mechanisms underpinning most cellular functions. Förster resonance energy transfer (FRET) is the gold standard for studying protein-protein interactions. Fluorescence lifetime imaging microscopy (FLIM) allows for a straightforward quantifica…
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Optimizing Nuclei Extraction & Counting for Single Cell Sequencing
46:44
46:44
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Single cell sequencing samples must be prepared to the highest possible standard. Discover reliable techniques to prepare and validate nuclei samples for single cell sequencing. In this episode, learn how to perform essential quality control checks, including a quick and reliable method for quantifying nuclei, measuring extraction efficiency, and v…
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How to Effectively Communicate Your Research
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Join Kate Christian as she demystifies the process of effectively communicating your research in the latest episode of Listen In. She describes the impact of research communication in and out of the lab and provides some guides to explaining your research in various contexts, such as lab tours, community visits, and other public engagements. As wel…
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Imaging GPCR Activity: Unravelling Mechanisms from Molecules to Morphology
1:08:22
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Precise control of cellular communication and signaling is crucial for every physiological system, with G protein-coupled receptors (GPCRs, the largest family of signaling receptors) playing a central role as essential molecular sensors of a cell’s environment. Because of their diverse physiological and pathophysiological roles, GPCRs are also majo…
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Critical Analysis Of Research Papers: Practical Examples
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42:45
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As a researcher, one of the most crucial skills to develop is the ability to perform an efficient yet thorough review of manuscripts. Manuscript reviewing requires specific skills and knowledge to properly assess published literature. By reading and reviewing papers, researchers can develop and hone these skills, which will help them identify and h…
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New TauSTED Tools for Gentle Live Imaging at Nanoscale Resolution
1:04:16
1:04:16
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This episode of Listen In showcases the latest tools and developments for multiplex STED imaging of living specimens at nanoscale resolution. Confocal imaging is a fundamental tool for studying the complex interplay of biomolecules, molecular machines, and higher-order cellular structures owing to its optical sectioning, sensitivity, and temporal a…
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Finding The Ideal Hook for Your Science Story
37:25
37:25
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Your research is important, but it can be hard to explain to others why it's important. In this episode of Listen In, you'll learn to find the ideal hook for your science story. This trick, borrowed from journalism, involves taking a step back and thinking about all the aspects of your research and where they might overlap with other topics that at…
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Culture Media Preparation: Contamination Control and Retention of Critical Components
52:37
52:37
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Contamination poses a significant risk in cell culture, jeopardizing research outcomes and incurring costly setbacks. This episode of Listen In gives you valuable insights into often overlooked areas of your filtration processes and will help you safeguard your sensitive cell cultures. Explore the critical importance of sterile filtration in divers…
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Automate Your Pipetting — Unleash Your Potential
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Despite the growing number of new technologies and automated instruments, routine liquid handling is still a manual process in many labs. The trend towards automation is gaining importance, not only with complex applications, such as NGS library preparation but also with simple yet repetitive liquid handling steps where automated pipetting makes se…
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Cellular Crosstalk in Neurodevelopmental Disorders
1:03:20
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Inspired by mutations associated with neurodevelopmental disorders, Prof. Dr. Silvia Cappello’s group at the Ludwig Maximilian University of Munich aims to understand the role of extracellular mechanisms essential for the correct development of the human brain. Their research focuses on intrinsic and extrinsic mechanisms contributing to the formati…
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End-point PCR Set-up and Optimization: Best Practice
57:59
57:59
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In this episode of Listen In, master your PCR set-up for unparalleled results. Check out this enlightening discussion of the crucial factors that influence the outcome of PCR experiments and discover the parameters you can optimize for better and more consistent PCR results. You will get reliable advice directly from PCR experts at Eppendorf on key…
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Approaches for More Transparent, Rigorous, and Reproducible Research
48:52
48:52
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Improving open science and research reproducibility is both a technical and a social challenge. But how do we do it? One way is to design, conduct, and report research that can maximize the impact of our individual and collective investment in research. Plus, changing the research culture to prioritize 'getting it right' over 'getting it published'…
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Confirmation Bias and The Scientific Method II: Applying the Scientific Method
1:18:26
1:18:26
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For many researchers, modern science has become about getting results rather than asking questions. Results mean publications, and publications mean more funding. However, results are not the objective of the scientific method, which involves proposing an answer to a question, designing experiments to determine whether the proposed answer is true o…
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Confirmation Bias and The Scientific Method I: How to Avoid Confirmation Bias
56:54
56:54
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For many researchers, modern science has become about getting results rather than asking questions. Results mean publications, and publications mean more funding. However, results are not the objective of the scientific method, which involves proposing an answer to a question, designing experiments to determine whether the proposed answer is true o…
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Cryo-tomography to Volume EM: Explore with Hydra Bio Plasma-FIB
1:00:54
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Volume electron microscopy or volume EM (vEM) was named one of Nature's Technologies to Watch in 2023. The new Thermo Scientific Hydra Bio PFIB integrates advanced cryo-TEM lamella preparation to volume EM workflows. In this episode of Listen In, see how the new Thermo Scientific Hydra Bio Plasma-Focused Ion Beam (PFIB) extends cryo technology to v…
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The 12Rs Framework: Enabling High-quality Humane Research Involving Animals
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In this episode of Listen In, see how emerging and global challenges of conducting ethical animal research have led to the 12Rs Framework to guide humane experimental techniques in animal studies and other research areas. The Principles of Humane Experimental Technique, the 3Rs (Replacement, Refinement, and Reduction), have been the mainstay of hum…
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Elevate Your Research with Spatial Insights
57:14
57:14
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In this episode of Listen In, learn more about how the convergence of AI technology and scientific discovery can revolutionize our understanding of biology and disease. Generating insights into tissue microenvironments is crucial to our understanding of normal and abnormal tissue development, such as during cancer progression. Spatial biology metho…
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Unleash New Biological Insights with Innovative Glycoanalytics
53:21
53:21
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Glycosylation is a prevalent modification of proteins known to play diverse roles across a wide range of biological processes. While glycoproteins are widely recognized as key molecular features involved in human health and disease, the analysis of protein glycosylation has historically been hampered by a lack of efficient and simple methods to cha…
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Contamination and Recovery of PFAS in Analytical Methods Requiring Filter Membranes
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Liquid chromatography-mass spectrometry (LC-MS) is a crucial technique in many life sciences, including proteomics, metabolomics, pharmaceutical analysis, clinical diagnosis, and forensic science. In this episode of Listen In, elevate your analysis and liquid chromatography. Learn what consumables, materials, and reagents can harm your LC-MS data f…
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Label-free 3D Live Cell Imaging and Quantification using Holotomography
1:02:03
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Holotomography has emerged as a helpful tool for imaging live specimens without additional pre-treatment, such as fixation, fluorescence labeling, and excitation. It can achieve long-term three-dimensional observations of live specimens for weeks without cellular damage caused by photoactivation. The high resolution (under 150 nm lateral) achieved …
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Back to Buffer Basics: Everything You Need to Know About Buffers
57:09
57:09
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Buffers are critical to nearly all our experiments. Poorly prepared buffers lead to failed Western blots, poor protein yields, flat binding curves—and wasted time. Understanding why you need buffers, knowing how to choose the right one, and troubleshooting buffer issues are fundamental skills you can apply to make all your experiments work better—a…
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How to Write a Research Paper IV: Understanding the Publication Process
49:24
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You have written a research paper. What now? Your research can only have an impact if someone reads it. So, you must submit your article to a journal for publication. In this episode of Listen In, learn the nuts and bolts of scientific publication with a step-by-step tour of the whole process. Explore how to select the right journal and what tools …
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How to Write a Research Paper III: Common Problems and How to Overcome Them
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The best way to solve a problem is to avoid it in the first place. While journals can and do reject articles for scientific reasons, they also reject articles for structural issues. By taking a few simple steps, you can learn how to circumvent these difficulties for a smooth publication process. In this episode of Listen In, we'll go through some o…
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How to Write a Research Paper II: The Anatomy
1:01:54
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All research papers have certain elements in common: Introduction, Methods, Results, and Conclusion. How can you structure your introduction to best frame the research question for your readers? Must the methods section read like a laundry list of reagents? Can you be dramatic in the results without losing objectivity? Is there any room in the conc…
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How to Write a Research Paper I: Telling Your Story
1:08:27
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Rarely do we consider writing a scientific paper as a creative exercise. But what if that could change? The best way to communicate anything is through stories. The scientific process has all the elements inherent in storytelling: a rich background, a problem or conflict to solve, and a resolution. In this episode of Listen In, we will reframe the …
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Using Antisense to Characterize Nonsense: A Mouse Model of Progranulin-Deficient Dementia
42:09
42:09
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Transgenic mouse models are important tools for testing therapeutic strategies. In this episode of Listen In, discover the benefits and essential considerations when characterizing and using transgenic mouse models to test therapeutic approaches to treat neurodegenerative diseases. Plus, explore a knock-in mouse model of frontotemporal dementia tha…
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Visualizing Membrane Dynamics by Electron Microscopy
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Explore how the latest electron microscopy techniques and volume imaging can capture fast, dynamic membrane and vesicle remodeling events during synaptic transmission at the ultrastructural level. Neurons communicate at specialized junctions, or synapses, via chemical messengers called neurotransmitters. Within a millisecond of neuronal stimulation…
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Benefits of Imaging Data in Flow Cytometry Experiments: A Panel Discussion
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41:43
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Experience the transformative power of unbiased, data-driven cell analysis with access to over 25 image-derived label-free parameters, empowering you to easily assess sample quality, optimize gating strategies, and validate rare events. In this episode, learn how automated image analysis combined with flow cytometry can help improve data accuracy a…
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Jump into 3D Organoids: Basic Culture Techniques and Advanced Applications
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Organoids are three-dimensional in vitro cultures derived directly from patient tissues or induced pluripotent stem cells (iPSC) and embryonic stem cells. They self-organize to generate structures that resemble the tissue of origin and recapitulate the key physiological functions of the parental organ. In this episode of Listen In, explore the bene…
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Ethical Research Practice III: Image Manipulation – What's Ok, and What's Not
59:36
59:36
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As a scientific researcher, you must present your work in an unbiased, original, and representative way. Without due care and attention, it is easy to drift over the boundary of what is acceptable and slip into habits that are unacceptable within the scientific community. This episode of Listen In with Cristy Gelling explores image manipulation. Ma…
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Ethical Research Practice II: Reporting Negative Results and Outliers
48:41
48:41
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As a scientific researcher, you must present your work in an unbiased, original, and representative way. Without due care and attention, it is easy to drift over the boundary of what is acceptable and slip into habits that are unacceptable within the scientific community. This episode of Listen In with Karen O'Hanlon Cohrt explores publishing negat…
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Ethical Research Practice I: Plagiarism – What It Is and How to Avoid It
59:54
59:54
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As a scientific researcher, you must present your work in an unbiased, original, and representative way. Without due care and attention, it is easy to drift over the boundary of what is acceptable and slip into habits that are unacceptable within the scientific community. This episode of Listen In with Karen O'Hanlon Cohrt defines plagiarism and pr…
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Everyday Culture Practice: Improving Reproducibility in Cell Culture
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Eukaryotic cell cultures respond to the slightest influence. Apart from the risk of contamination, minimal changes in cultivation parameters can affect their viability, growth, and cell metabolism. In this episode of Listen In, discover the measures you can take to improve the reproducibility of your experiments by incorporating Good Cell Culture P…
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Precision and Efficiency at Your Fingertips: Why Electronic Pipettes are the Future of Pipetting
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Many scientists see the added value in using electronic pipettes and have already upgraded their pipette fleet. The benefits include: • Reduced strain on thumbs and arms by improving pipetting ergonomics. • Increased reproducibility of experiments with motor-operated pipetting cycles. • Easy and efficient handling of high sample throughput. In this…
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Introduction to Liquid Handling for Next-generation Sequencing Workflows
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Next-generation sequencing (NGS) has revolutionized the ‘omics’ era, with the rapid decoding of massive DNA sequences, supporting the investigation into various biological processes in human disease, genetic inheritance, immunity, cancer, and others. But generating high-quality NGS libraries is a labor-intensive process that requires experience, pr…
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Essentials in Centrifugation—Better Safe than Sorry!
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Centrifugation is the cornerstone of lab life, used in nearly every workflow, be it a routine task like pelleting cells or a dedicated assay for an important biomolecular target. That's why it's such a pain when they break down. But just because we use centrifuges every day doesn't mean there aren't important technical aspects to consider or no par…
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Ten Common Statistical Mistakes to Watch out for When Writing or Reviewing a Manuscript
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51:32
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Some scientific papers are unbelievable as they showcase experimental excellence that we did not think possible. These papers astound us with their groundbreaking findings and meticulous methodologies. However, alongside these unbelievably good papers, there are papers that are unbelievable due to the pervasive presence of statistical mistakes that…
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Your ultra-low temperature (ULT) freezer is not just a storage room for samples; it is your assurance for long-term scientific success and guards the results of your work. Therefore, every researcher should understand the importance of ULT freezers, know how to choose a reliable one, understand best practices for sample management, and have clear g…
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Reproducibility: Mastering the Art of Pipetting
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Have you ever transferred glycerol or ethanol with a pipette? We all know how hard it is, and we've all been dubious about the accuracy of our final volumes. While you probably realize that your liquid type and pipetting technique can influence your analysis results, you might be less sure about what pipettes to use when your assays involve challen…
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Real-time IntraVital Microscopy (IVM): In Vivo Cellular-level Imaging of Internal Organs in a Live Animal
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Intravital imaging of cellular dynamics in a natural physiological microenvironment can provide unprecedented insights into the dynamic pathophysiology of diseases. In this episode of Listen In, get a demonstration of the latest technology to image inside the cells of live animals. Watch real-time multicolor sub-micron resolution image acquisition …
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Errors and Misconduct in Biomedical Research
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What are the motives behind science misconduct? How is data manipulated to evidence dubious results? And do you think you can spot edited and faked data? Any fraud in science is too much, but the reasons for it are complex. In this episode of Listen In, join forensics detective Elisabeth Bik and explore the answers to these questions. See the work …
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Garbage In, Garbage Out: Sample Prep for Flow Cytometry
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What's more disappointing than running your flow cytometry sample to discover it's rubbish? Especially considering time on cytometers can be precious, even more so if you've booked onto an in-demand one at a busy core facility. You've done all the work and all the waiting—for nothing. If this scenario sounds familiar, this episode of Listen In is f…
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Consistent, Error-Free Writing: Tips and Tricks for Time-starved Scientists
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Scientific writing is an art that requires as much attention and proficiency as your hands-on lab work. When done right, it can significantly contribute to your scientific endeavors. But, if not taken seriously, inconsistent language, formatting, and terminology can hinder your work's clarity and distract from your remarkable scientific findings. I…
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Lab Meetings Live: Career Paths for Bioscientists
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Are you an early career scientist looking for guidance on your career path? Or you're a seasoned researcher considering a career change and want to know what's out there. We've brought together a panel of experts and bioscientists with a wide range of experience to share their insights and help you carve out the best career possible. Get advice and…
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Breaking the Ice: Freeze Fracture for Cryo SEM
1:16:16
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Freeze fracture is a powerful technique that enables imaging of internal structures of cells and tissues at the nanoscale level by freezing samples at ultra-low temperatures and then fracturing them along natural weak points. These structures can be imaged without dehydration or distortion using cryo-SEM, providing high-resolution images with great…
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Practical Examples of Critical Analysis of Manuscripts
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42:22
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As a researcher, one of the most crucial skills to develop is the ability to perform a thorough review of manuscripts. Manuscript reviewing is a vital job that requires specific skills and knowledge to properly assess published literature. By reading and reviewing papers, researchers can develop and hone these skills, which will help them identify …
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1
Using Both the Right and Left Parts of Your Brain to Get the Perfect Job
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Have you ever wondered what other jobs are ideal matches for your analytic and artistic skills? Are you looking for an exciting new career in marketing, or have you ever wondered if it was for you? In this episode of Listen In, discover how the assets that make you a great scientist could also make you a great marketer in today's data-driven market…
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How to Prepare a Winning Grant Proposal – Part 3: Resubmission
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In the third part of our grant writing tutorial series, you’ll learn how to process a grant review and take the next steps. Dr. Seigel shares how to read and interpret a summary statement, ways to address critiques, and when you shouldn't resubmit. You'll hear real-world examples from previous grants to give you a flavor of what you can expect. Wat…
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How to Prepare a Winning Grant Proposal – Part 2: Nuts and Bolts
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In the second part of our grant writing tutorial series, you’ll learn how to get the attention and respect of the reviewers by presenting your ideas in the best possible light. Dr. Seigel shares essential elements of writing the research plan section of your proposal, such as the essential items to include in your research plan, how to write a comp…
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How to Prepare a Winning Grant Proposal – Part 1: Getting Started
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Writing grant proposals can be daunting, especially when the stakes are high. Getting started is the first and potentially hardest step to writing a proposal that gets you funded. Join Dr. Gail Seigel, a highly experienced faculty member with over 25 years of grant-writing and grant-reviewing expertise, as she shares her wisdom on kickstarting the …
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